ESTABLISHING THE POSSIBILITIES OF USING THE WASTE SUBSTRATE AFTER GROWING LENTINULA EDODES
Abstract
In the context of modern food challenges to produce a wide range of protein-based foods, including functional and health foods and vegetarian food, artificial cultivation of mushrooms is attracting attention. Currently, the food industry in Ukraine is focused on the artificial cultivation of various types of mushrooms. Currently, the food industry in Ukraine is focused on the artificial cultivation of mushrooms of various species. In particular, this article will focus on mushrooms of the genus Lentinula edodes. After mushroom cultivation, large quantities of waste substrate remain, which is mostly the remains of lignocellulosic phytomass. Under current environmental rules and regulations, the issue of waste-free or closed-cycle production is becoming increasingly important. The aim of the study is to investigate the micro- and macroelement composition and general microbiological activity of the spent Lentinula edodes substrate in order to find ways to bioconvert this substrate and the possibility of its further use in the food industry, for organic farming or as a raw material for biofuel production. The content of micro- and macronutrients was determined using a half-beam photometer. To study the microbiological activity of the spent substrate after mushroom cultivation, aqueous extract and sterile pieces were used. For microbiological inoculation, meat-peptone agar, Slate-Bartley medium, and Chapek's medium were used as nutrient media. According to the results of the micro- and macroelemental and microbiological study on the possibility of using the spent Lentinula edodes substrate, a number of regularities and interactions were established, which opens up wide prospects for further research on the spent substrate after cultivation of Lentinula edodes with its subsequent purification and enrichment of food with the obtained dietary fibres, or the creation of new functional foods based on the obtained food prebiotics of cellulosic origin. The obtained results open up new areas of research on the search for biologically active components of the spent Lentinula edodes substrate in the food industry and in the agricultural sector.
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